Two Strains of Endophytic <i>Bacillus velezensis</i> Carrying Antibiotic-Biosynthetic Genes Show Antibacterial and Antibiofilm Activities Against Methicillin-Resistant <i>Staphylococcus aureus</i> (MRSA).

ABSTRACT: Methicillin-resistant Staphylococcus aureus (MRSA) is considered a priority pathogen causing high mortality that requires effective control measures. This study aimed to detect the presence of antibiotic-biosynthetic genes and to evaluate the anti-MRSA activity of two strains of endophytic Bacillus velezensis isolated from Archidendron pauciflorum. PCR-based screening showed that B. velezensis strains, such as DJ4 and DJ9 possessed six antibiotic-biosynthetic genes, namely MlnA , DhbE , BacD , DfnD, SrfA, and BaeR. According to the preliminary test conducted using disc-diffusion assay, metabolite extracts from these strains have anti-MRSA activity with clear zone diameters of 13.00 ± 0.82 mm, and 17.

SUBMITTER: Priyanto JA 

PROVIDER: S-EPMC11645368 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature