Insights into substrate recognition and export tunnel preferences in the efflux transporter AcrB.

ABSTRACT: In Escherichia coli AcrB is a major multidrug exporter, which confers the bacterium resistance to many antibiotics with diverse structural and chemical proprieties. Studies have identified three possible tunnels (or channels) within AcrB that different substrates use before reaching the distal pocket, from which they are subsequently extruded. Recently, we reported that mutations in the AcrB gate loop may affect the conformational change kinetics involved in substrate export rather than directly affecting molecular interactions with this loop, and we highlighted the distinct export tunnel preferences between erythromycin and doxorubicin. To further understand the gate loop's role in AcrB's export activity and the rationale behind substrate preferences among the three possible export tunnel

SUBMITTER: Makhamadjamonov F 

PROVIDER: S-EPMC11645668 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature