Evolution of a novel engineered tripartite viral genome of a torradovirus.

ABSTRACT: Viruses in the Secoviridae include monopartite and bipartite genomes, suggesting the possibility to study members of this family to experimentally address evolutionary transitions resulting in multipartitism. Torradoviruses are bipartite members of the family Secoviridae characterized by a genus-specific 5' open reading frame, named P21, encoded by RNA2. Here, in a study originally intended to verify if P21 can function in trans, we attempted to provide P21 from a third P21-expressing construct under control of the 35S promoter and containing the 5'- and 3'-untranslated regions (UTRs) of wild-type (WT) RNA2. When this construct was combined with an RNA2 with a complete deletion of the P21 coding region we verified that the P21 provided in trans cannot immediatel

SUBMITTER: Turina M 

PROVIDER: S-EPMC11646122 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature