The Ribosomal Operon Database: A Full-Length rDNA Operon Database Derived From Genome Assemblies.

ABSTRACT: Current rDNA reference sequence databases are tailored towards shorter DNA markers, such as parts of the 16/18S marker or the internally transcribed spacer (ITS) region. However, due to advances in long-read DNA sequencing technologies, longer stretches of the rDNA operon are increasingly used in environmental sequencing studies to increase the phylogenetic resolution. There is, therefore, a growing need for longer rDNA reference sequences. Here, we present the ribosomal operon database (ROD), which includes eukaryotic full-length rDNA operons fished from publicly available genome assemblies. Full-length operons were detected in 34.1% of the 34,701 examined eukaryotic genome assemblies from NCBI. In most cases (53.1%), more than one operon variant was detected, which can be due to intragen

SUBMITTER: Krabberod AK 

PROVIDER: S-EPMC11646303 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature