Identification of differentially expressed genes in human testis biopsies with defective spermatogenesis.

ABSTRACT:

Purpose

Sperm morphology and motility are major contributors to male-factor infertility, with many genes predicted to be involved. This study aimed to elucidate differentially expressed transcripts in human testis tissues of normal and abnormal spermatogenesis that could reveal new genes that may regulate sperm morphology and function.

Methods

Human testis biopsies were collected from men with well-characterized phenotypes of normal spermatogenesis, spermatid arrest, and Sertoli cell-only phenotype, and transcriptional differences were quantified by RNA-sequencing (RNA-Seq). Differentially expressed genes (DEGs) were filtered based on predominant expression in spermatids and gene functional annotations relevant to sperm morphology and motility. Selected 10 DEGs were validate

SUBMITTER: Kothalawala SD 

PROVIDER: S-EPMC11646353 | biostudies-literature | 2024 Jan-Dec

REPOSITORIES: biostudies-literature