Activation characteristics of Ty3-retrotransposons after spaceflight and genetic stability of insertion sites in rice progeny.

ABSTRACT:

Introduction

The space environment is mutagenic and may induce genomic and phenotypic variations. Exploring the changes in transposon activity in the rice genome under space radiation is of great significance.

Methods

To analyze the activation characteristics of Ty3-retrotransposons and genetic stability of insertion sites in rice progeny after spaceflight, seeds of Nipponbare, DN416, and DN423 were exposed on board the SJ-10 recoverable satellite for 12.5 days. The differential methylation and transcription levels of Ty3-retrotransposons in the genome of Nipponbare's F0 generation after spaceflight, as well as the genetic stability of Ty3-retrotransposon insertion sites in DN416 and DN423 from F3 to F5 generations, was analyzed.

Results

The study found that the retro

SUBMITTER: Yang Q 

PROVIDER: S-EPMC11646775 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature