Quantifying conformational changes in the TCR:pMHC-I binding interface.

ABSTRACT:

Background

T cells form one of the key pillars of adaptive immunity. Using their surface bound T cell antigen receptors (TCRs), these cells screen millions of antigens presented by major histocompatibility complex (MHC) or MHC-like molecules. In other protein families, the dynamics of protein-protein interactions have important implications for protein function. Case studies of TCR:class I peptide-MHCs (pMHC-Is) structures have reported mixed results on whether the binding interfaces undergo conformational change during engagement and no robust statistical quantification has been done to generalise these results. Thus, it remains an open question of whether movement occurs in the binding interface that enables the recognition and activation of T cells.

Methods

In this work,

SUBMITTER: McMaster B 

PROVIDER: S-EPMC11646856 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature