Genome sequencing of <i>Escherichia coli</i> phage <i>UFJF_EcSW4</i> reveals a novel lytic <i>Kayfunavirus</i> species.

ABSTRACT: The Escherichia coli phage UFJF_EcSW4 was isolated from polluted stream water and showed clear lysis plaques on the host, measuring 0.67 ± 0.43 mm, with a titer of 9.57 ± 0.23 log PFU/ml. It demonstrated a very narrow host range, infecting only its host. Additionally, it has a short latent period of 9 min, a burst size of 49 PFU/infected cell, and stability over a wide range of pH, temperature, and free residual chlorine. The phage has a double-stranded DNA genome spanning 40,299 bp, with a GC content of 49.87% and short-direct terminal repeats (DTR) sequences of 286 bp. The UFJF_EcSW4 genome contains 55 genes, organized into functional modules with a unidirectional arrangement, regulated by 22 promoters (three from the phage and 19 from the host) and three Rho-indepen

SUBMITTER: Vidigal PMP 

PROVIDER: S-EPMC11646959 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature