Microfluidic impedance flow cytometer leveraging virtual constriction microchannel and its application in leukocyte differential.

ABSTRACT: Microfluidic impedance flow cytometry has been widely used in leukocyte differential and counting, but it faces a bottleneck due to the trade-off between impedance detection throughput and sensitivity. In this study, a microfluidic impedance flow cytometer based on a virtual constriction microchannel was reported, in which the virtual constriction microchannel was constructed by crossflow of conductive sample and insulated sheath fluids with underneath micro-electrodes for impedance measurements. Compared to conventional mechanical constriction microchannels, this virtual counterpart could effectively avoid direct physical contact between cells and the microchannel walls to maintain high throughputs, and significantly reduce the volume of the impedance detection region for sensitivity impr

SUBMITTER: Wang M 

PROVIDER: S-EPMC11647040 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature