Proteomic dataset of knockdown of AKT3 on protein expression and function in female germline stem cells.

ABSTRACT: Female germline stem cells (FGSCs) are adult stem cells capable of self-renewal and differentiation into mature oocytes. AKT3, a member of the AKT kinase family, plays crucial roles in multiple cellular processes, such as proliferation, migration, and apoptosis. However, the mechanism by which AKT3 affects the development of FGSCs is poorly understood. We performed 4D-data-independent acquisition (DIA) Quantitative Proteomics on mouse FGSCs in which AKT3 was knocked down using a lentivirus and on control FGSCs. Based on the raw DIA data, coupled with database searches and data filtering, we identified 46,260 peptides, including 45,821 unique peptides, corresponding to 6849 identified proteins and 6697 comparable proteins. These identified proteins were functionally annotated using Gene Ont

SUBMITTER: Shen Y 

PROVIDER: S-EPMC11647134 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature