The effects of age and sex on reference intervals for cobalamin, homocysteine, and serum and urinary methylmalonic acid in healthy adult dogs.

ABSTRACT:

Background

In dogs, data on reference intervals (RIs) for cobalamin, markers of metabolism (markersB₁₂met), age and sex effects are limited.

Hypothesis/objectives

Establish RI for serum cobalamin, homocysteine, and methylmalonic acid (sMMA) concentrations, urinary methylmalonic acid-to-creatinine ratio (uMMA:crea), and determine effects of sex and age.

Methods

Prospective study using healthy dogs (1-10 years). Cobalamin and markersB₁₂met were determined using chemiluminescence immunoassay (cobalamin) and liquid chromatography/tandem mass spectrometry (homocysteine, sMMA, uMMA:crea). In dogs with outlying data, changes in health, markersB₁₂met, and onset of gastrointestinal signs were reevaluated after 9-15 months.

Results

Twelve

SUBMITTER: Proksch AL 

PROVIDER: S-EPMC11647175 | biostudies-literature | 2025 Jan-Feb

REPOSITORIES: biostudies-literature