Customised 96-ocular TaqMan (iCAM) microarray PCR card for rapid diagnosis of microbial keratitis.

ABSTRACT:

Aim

To validate the diagnostic performance of a custom 96-micro-organism TaqMan PCR card (iCAM) for microbial keratitis (MK) from a single corneal epithelial sample.

Methods

Patients over the age of 18 referred to Cambridge University Hospital with MK were recruited in this single-site prospective cohort study between September 2021 and January 2023. An ocular-specific, customised microarray card (iCAM) was constructed according to primer and probe nucleotide sequences developed in our department to detect bacteria, viruses, Acanthamoeba and fungi commonly implicated in MK using a single corneal epithelial sample. Part of the corneal epithelial sample was taken for conventional cultures per local protocol, followed by iCAM array. Microbial detection rate and positive

SUBMITTER: Yang Y 

PROVIDER: S-EPMC11647283 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature