Rho and riboswitch-dependent regulations of mntP gene expression evade manganese and membrane toxicities.

ABSTRACT: The trace metal ion manganese (Mn) in excess is toxic. Therefore, a small subset of factors tightly maintains its cellular level, among which an efflux protein MntP is the champion. Multiple transcriptional regulators and a manganese-dependent translational riboswitch regulate the MntP expression in Escherichia coli. As riboswitches are untranslated RNAs, they are often associated with the Rho-dependent transcription termination in bacteria. Here, performing in vitro transcription and in vivo reporter assays, we demonstrate that Rho efficiently terminates transcription at the mntP riboswitch region. Excess manganese activates the riboswitch, restoring the coupling between transcription and translation to evade Rho-dependent transcription termination partially. RT-PCR and Western blot exper

SUBMITTER: Prakash A 

PROVIDER: S-EPMC11647493 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature