Structural profiles of the full phagocyte NADPH oxidase unveiled by combining computational biology and experimental knowledge.

ABSTRACT: The phagocyte NADPH oxidase (NOX2) is an enzyme, crucial for innate immune defense, producing reactive oxygen species necessary for pathogen destruction. Its activation requires the assembly of soluble proteins (p47^phox, p40^phox, p67^phox, and Rac) with the membrane-bound flavocytochrome b₅₅₈ (cytb₅₅₈). We combined circular-dichroism analyses, with decades of experimental data, to filter structural models of the NADPH oxidase complex generated by the artificial intelligence program AlphaFold2 (AF2). The predicted patterns tend to closely resemble the active states of the proteins, as shown by the compact structure of the cytb₅₅₈, whose dehydrogenase domain is stabilized closer to the membrane. The modeling of the interaction

SUBMITTER: Aimeur S 

PROVIDER: S-EPMC11647612 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature