Immobilizing calcium-dependent affinity ligand onto iron oxide nanoparticles for mild magnetic mAb separation.

ABSTRACT: Current downstream processing of monoclonal antibodies (mAbs) is limited in throughput and requires harsh pH conditions for mAb elution from Protein A affinity ligands. The use of an engineered calcium-dependent ligand (Z_Ca) in magnetic separation applications promises improvements due to mild elution conditions, fast processability, and process integration prospects. In this work, we synthesized and evaluated three magnetic nanoparticle types immobilized with the cysteine-tagged ligand Z_Ca-cys. Ligand homodimers were physically immobilized onto bare iron oxide nanoparticles (MNP) and MNP coated with tetraethyl orthosilicate (MNP@TEOS). In contrast, Z_Ca-cys was covalently and more site-directedly immobilized onto MNP coated with (3-glycidyloxypropyl)trimeth

SUBMITTER: Zimmermann I 

PROVIDER: S-EPMC11647653 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature