DNA Hybridization Kinetics Observed at the Single-Molecule Level Using Graphene Near-Field Effects.

ABSTRACT: We present the development of an advanced sensing platform using a monolayer of graphene functionalized with fluorophore-labeled DNA hairpins to detect the kinetics of single hairpins during the hybridization reaction. The near-field photonic effects of graphene induce a distance-dependent quenching effect on the attached fluorescent labels, resulting in distinct optical signals in response to axial displacements resulting from DNA hybridization. Employing a wide-field Total Internal Reflection Fluorescence (TIRF) optical setup coupled with a sensitive Electron-Multiplying Charge-Coupled Device (EM-CCD) camera, we successfully detected fluorescent signals of individual or a low number of individual DNA hairpins within a low-concentration environment DNA target (tDNA). These signals were us

SUBMITTER: Soares MM 

PROVIDER: S-EPMC11647878 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature