AAV-regulated <i>Serpine2</i> overexpression promotes hair cell regeneration.

ABSTRACT: Inner ear hair cell (HC) damage is irreversible in mammals, but it has been shown that supporting cells (SCs) have the potential to differentiate into HCs. Serpine2, a serine protease inhibitor, encodes protease nexin 1, and this has been suggested to be a factor that promotes HC regeneration. In this study, we overexpressed Serpine2 in inner ear SCs cultured in two- and three-dimensional systems using the adeno-associated virus-inner ear (AAV-ie) vector, which promoted organoid expansion and HC differentiation. Overexpression of Serpine2 in the mouse cochlea through the round window membrane (RWM) injection promoted SC proliferation and HC regeneration, and the regenerated HCs were found to be derived from Lgr5⁺ SCs. Regenerated HCs have electrophysiologica

SUBMITTER: Sun Q 

PROVIDER: S-EPMC11648234 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature