Substrate specificity and kinetic mechanism of 3β-hydroxy-Δ⁵-C₂₇-steroid oxidoreductase.

ABSTRACT: Cholesterol is a key sterol whose homeostasis is primarily maintained through bile acid metabolism. Proper bile acid formation is vital for nutrient and fat-soluble vitamin absorption and emulsification of lipids. Synthesis of bile acids occurs through two main pathways, both of which rely on 3β-hydroxy-Δ⁵-C₂₇-steroid oxidoreductase (HSD3B7) to begin epimerization of the 3β hydroxyl of cholesterol into its active 3α conformation. In this sequence, HSD3B7 catalyzes the dehydrogenation of the 3β-hydroxy group followed by isomerization of the Δ⁵-cholestene-3-one. These reactions are some of the many steps that transform cholesterol for either storage or secretion. HSD3B7 has distinct activity from other 3β-HSD family members leaving significant gaps in our und

SUBMITTER: Gardner SM 

PROVIDER: S-EPMC11648244 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature