Oxidative stress controls lncRNA-mediated sow granulosa cell functions in a FoxO1-dependent manner.

ABSTRACT:

Background

Oxidative stress (OS) is involved in low female fertility by altering multi-omics such as the transcriptome, miRome, and lncRNome in follicular cells and follicular fluid. However, the mechanism by which OS affects multi-omics dynamics remains largely unknown. Here, we report that OS induces lncRNome dynamics in sow granulosa cells (sGCs), which is partially dependent on the transcription factor activity of its effector, FoxO1.

Results

A total of 2,283 putative FoxO recognition elements (FREs) were identified in the promoters of 394 lncRNAs, accounting for 91.20% (394/432) of the lncRNAs regulated by OS. ChIP and reporter assays showed that the effector FoxO1 mediated OS regulation of lncRNA transcription in a transcription factor activity-dependent manner. In sGC

SUBMITTER: Sheng W 

PROVIDER: S-EPMC11648296 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature