Assessment of NanoString technology as a tool for profiling circulating miRNA in maternal blood during pregnancy.

ABSTRACT:

Aim

Circulating maternal MicroRNA (miRNA) is a promising source of biomarkers for antenatal diagnostics. NanoString nCounter is a popular global screening tool due to its simplicity and ease of use, but there is a lack of standardisation in analysis methods. We examined the effect of user-defined variables upon reported changes in maternal blood miRNA during pregnancy.

Methods

Total RNA was prepared from the maternal blood of pregnant and control rats. miRNA expression was profiled using Nanostring nCounter. Raw count data were processed using nSolver using different combinations of normalisation and background correction methods as well as various background thresholds. A panel of 14 candidates in which changes were supported by multiple analysis workflows was selected for

SUBMITTER: Adamova P 

PROVIDER: S-EPMC11648433 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature