Transfer of <i>miR-100</i> and <i>miR-125b</i> increases 3D growth and invasiveness in recipient cancer cells.

ABSTRACT:

Aim

Extracellular communication via the transfer of vesicles and nanoparticles is now recognized to play an important role in tumor microenvironment interactions. Cancer cells upregulate and secrete abundant levels of miR-100 and miR-125b that can alter gene expression in donor and recipient cells. In this study, we sought to identify targets of miR-100 and miR-125b and conclusively demonstrate that microRNAs (miRNAs) can be functionally transferred from donor to recipient cells.

Methods

To identify targets of miR-100 and miR-125b, we used bioinformatic approaches comparing multiple colorectal cancer (CRC) cell lines, including knockout lines lacking one or both of these miRNAs. We also used spheroid and 3D growth conditions in colla

SUBMITTER: Nelson HM 

PROVIDER: S-EPMC11648436 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature