Heme metabolism in Strigomonas culicis: Implications of H₂O₂ resistance induction and symbiont elimination.

ABSTRACT: Monoxenous trypanosomatid Strigomonas culicis harbors an endosymbiotic bacterium, which enables the protozoa to survive without heme supplementation. The impact of H₂O₂ resistance and symbiont elimination on intracellular heme and Fe²⁺ availability was analyzed through a comparison of WT strain with both WT H₂O₂-resistant (WTR) and aposymbiotic (Apo) protozoa. The relative quantification of the heme biosynthetic pathway through label-free parallel reaction monitoring targeted mass spectrometry revealed that H₂O₂ resistance does not influence the abundance of tryptic peptides. However, the Apo strain showed increased coproporphyrinogen III oxidase and ferrochelatase levels. A putative ferrous iron transporter, homo

SUBMITTER: Bombaca ACS 

PROVIDER: S-EPMC11648444 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature