Assessing extracellular vesicles from bovine mammary gland epithelial cells cultured in FBS-free medium.

ABSTRACT:

Aim

Mammary gland extracellular vesicles (EVs) are found in both human and livestock milk. Our knowledge of the role of EVs in the mammary gland development, breast cancer and mastitis derives mainly from in vitro cell culture models. However, a commonly shared limitation is the use of fetal bovine serum (FBS) as a supplement, which naturally contains EVs. For this reason, the purpose of the study was to evaluate novel tools to investigate mammary gland EVs in vitro and in a FBS-free system.

Methods

Primary bovine mammary epithelial cells (pbMECs) and a mammary gland alveolar epithelial cell line (MAC-T) were cultured in a chemically defined EV-free medium. To find a reliable EV isolation protocol from a starting cell conditioned medium (10 mL), we compared eig

SUBMITTER: Silvestrelli G 

PROVIDER: S-EPMC11648452 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature