The isolation of VCAM-1⁺ endothelial cell-derived extracellular vesicles using microfluidics.

ABSTRACT: Background: Vascular cell adhesion molecule-1 (VCAM-1⁺) endothelial cell-derived extracellular vesicles (EC-EVs) are augmented in cardiovascular disease, where they can signal the deployment of immune cells from the splenic reserve. Endothelial cells in culture activated with pro-inflammatory tumor necrosis factor-α (TNF-a) also release VCAM-1⁺ EC-EVs. However, isolating VCAM-1⁺ EC-EVs from conditioned cell culture media for subsequent in-depth analysis remains challenging. Aim: We utilized the extracellular vesicles (EV) microfluidics herringbone chip (^EVHB-Chip), coated with anti-VCAM-1 antibodies, for selective capture of VCAM-1⁺ cells and EC-EVs. Methods and Results: Engineered EA.hy926 endothelial cells overex

SUBMITTER: Akbar N 

PROVIDER: S-EPMC11648473 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature