Substrate stiffness modulates extracellular vesicles' release in a triple-negative breast cancer model.

ABSTRACT:

Aim

The microenvironment effect on the tumoral-derived Extracellular Vesicle release, which is of significant interest for biomedical applications, still represents a rather unexplored field. The aim of the present work is to investigate the interrelation between extracellular matrix (ECM) stiffness and the release of small EVs from cancer cells. Here, we focus on the interrelation between the ECM and small extracellular vesicles (sEVs), specifically investigating the unexplored aspect of the influence of ECM stiffness on the release of sEVs.

Methods

We used a well-studied metastatic Triple-Negative Breast Cancer (TNBC) cell line, MDA-MB-231, as a model to study the release of sEVs by cells cultured on substrates of different stiffness. We have grown MDA-MB-231 cells on two

SUBMITTER: Senigagliesi B 

PROVIDER: S-EPMC11648499 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature