Comparison of miRNA cargo in human adipose-tissue <i>vs</i>. amniotic-membrane derived mesenchymal stromal cells extracellular vesicles for osteoarthritis treatment.

ABSTRACT:

Aim

Mesenchymal stromal cells (MSCs) emerged as a promising therapeutic option for osteoarthritis (OA) management, in particular those isolated from adipose tissue (hASCs) and amniotic membrane (hAMSCs). The cartilage protective and immunomodulatory features of hASCs and hAMSCs are ascribed to secreted factors, including extracellular vesicles (EVs) and embedded miRNAs. The purpose of this study was to compare EVs and shuttled miRNAs from both MSC types and discuss them in the frame of OA pathological tissues.

Methods

Human hASCs and hAMSCs were analyzed by flow cytometry. EVs were analyzed by flow cytometry, nanoparticle tracking analysis, and electron microscopy. High-throughput qRT-PCR miRNA data available in the literature were compared. Abundant miRNAs and their experim

SUBMITTER: Ragni E 

PROVIDER: S-EPMC11648501 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature