ABSTRACT: The effects of supplemental Zn within steroidal implant strategy on performance, carcass characteristics, trace mineral status, and muscle gene expression were tested in a 59-d study using 128 Angus-crossbred steers (492 ± 29 kg) in a 2 × 4 complete randomized design. Implant strategies included no implant (NoIMP) or Component TE-200 (TE200; Elanco, Greenfield, IN) administered on day 0. Zinc was supplemented at 0, 30, 100, or 150 mg Zn/kg dry matter (Zn0, Zn30, Zn100, Zn150, respectively) from ZnSO4. Steers were stratified by body weight (BW) to pens (n = 5 or 6 steers/pen) equipped with GrowSafe bunks (GrowSafe Systems Ltd., Airdrie, AB, Canada) and assigned treatments (n = 15, 16, or 17 steers/treatment). Cattle were weighed on days -1, 0, 18, and 59 with blood collected on days -1, 18,