Pathway and protein channel engineering of <i>Bacillus subtilis</i> for improved production of desthiobiotin and biotin.

ABSTRACT: Biotin (vitamin B₇) is a crucial cofactor for various metabolic processes and has significant applications in pharmaceuticals, cosmetics, and animal feed. Bacillus subtilis, a well-studied Gram-positive bacterium, presents a promising host for biotin production due to its Generally Recognized as Safe (GRAS) status, robust genetic tractability, and capacity for metabolite secretion. This study focuses on the metabolic engineering of B. subtilis to enhance biotin biosynthesis. Initially, the desthiobiotin (DTB) and biotin synthesis ability of different B. subtilis strains were evaluated to screen for suitable chassis cells. Subsequently, the titers of DTB and biotin were increased to 21.6 mg/L and 2.7 mg/L, respectively, by relieving the feedback

SUBMITTER: Wu Y 

PROVIDER: S-EPMC11648629 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature