Interspecies transcriptome profiles of human T cell activation and liver inflammation in a xenogeneic graft-versus-host disease model.

ABSTRACT:

Background

Xenogeneic transplantation induces acute graft-versus-host disease (aGvHD) and subsequent vital organ damage. Herein, we aimed to examine hepatic damage associated with aGvHD using histopathology and gene expression profiles.

Methods

A xenografic GvHD model was established by engrafting human peripheral blood mononuclear cells (PBMCs) into immunodeficient NOD-scid IL2Rγnull (NSG) mice after busulfan conditioning. NSG mice were assigned to groups treated with saline (S group) or a combination of busulfan and PBMCs (BP group). Histological lesions and RNA sequencing analysis of gene profiles in the BP group (GvHD model) were compared with those in the P group.

Results

Predominant T cell subsets (95 %) in the blood of the BP group were identified as cytotoxic

SUBMITTER: Jeong SY 

PROVIDER: S-EPMC11648781 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature