MiR-1458 is inhibited by low concentrations of Vitamin B6 and targets TBX6 to promote the formation of spermatogonial stem cells in Rugao Yellow Chicken.

ABSTRACT: Spermatogonial stem cells (SSCs) have vast application prospects in livestock and poultry production, genetic engineering, and medical research. However, the scarcity of SSCs and the complexity of their development limit the elucidation and verification of the mechanism of SSCs in vitro. Although miRNAs have been identified as critical players in germ cell development, upstream regulatory mechanisms by which miRNAs regulate SSCs formation are rarely reported. In this study, miR-1458, which was differentially expressed during SSCs formation, was selected by transcriptomic sequencing. We found that miR-1458, inhibited in an in vitro SSCs induction model, significantly upregulated the expression of germline marker genes (Cvh and integrin β1). Further analysis using Immunofluorescence and Flow

SUBMITTER: Geng Q 

PROVIDER: S-EPMC11648786 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature