Structure of yeast RAVE bound to a partial V₁ complex.

ABSTRACT: Vacuolar-type ATPases (V-ATPases) are membrane-embedded proton pumps that acidify intracellular compartments in almost all eukaryotic cells. Homologous with ATP synthases, these multisubunit enzymes consist of a soluble catalytic V₁ subcomplex and a membrane-embedded proton-translocating V_O subcomplex. The V₁ and V_O subcomplexes can undergo reversible dissociation to regulate proton pumping, with reassociation of V₁ and V_O requiring the protein complex known as RAVE (regulator of the ATPase of vacuoles and endosomes). In the yeast Saccharomyces cerevisiae, RAVE consists of subunits Rav1p, Rav2p, and Skp1p. We used electron cryomicroscopy (cryo-EM) to determine a structure of yeast RAVE bound to V₁. In the s

SUBMITTER: Wang H 

PROVIDER: S-EPMC11648922 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature