O-GlcNAcylation inhibits hepatic stellate cell activation.

ABSTRACT:

Background and aim

Protein O-GlcNAcylation is a critical post-translational modification regulating gene expression and fundamental cell functions. O-GlcNAc transferase (OGT) emerged as a key regulator of liver pathophysiology and disease. In this study, we aimed to evaluate the role of OGT in hepatic stellate cells (HSCs) and its consequent role in liver fibrosis.

Methods

Primary HSCs were isolated from C57/B6 mice. Cell morphology and α-SMA immunofluorescence staining were observed under scanning confocal microscope. Transcriptomic profile was evaluated by RNAseq (Illumina). Promoter activity was examined by luciferase and β--Galactosidase reporter assays. Liver fibrosis mouse models were induced either by intraperitoneal injection of CCl₄ at 3 times/week for 4

SUBMITTER: Li R 

PROVIDER: S-EPMC11648924 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature