Exploring T Cell and NK Cell Involvement in Ankylosing Spondylitis Through Single-Cell Sequencing.

ABSTRACT: To uncover the complex immune mechanisms driving inflammation in ankylosing spondylitis and lay the groundwork for identifying new therapeutic targets and innovative approaches, we conducted 10× single-cell sequencing on bone marrow cell samples collected from the vertebrae of three AS patients and three non-AS patients. Using single-cell sequencing data, we analysed the expression of differentially expressed genes (DEGs) by comparing AS patients with non-AS patients. Key genes among the related DEGs were identified through protein-protein interaction networks and hub gene screening and further validated using immunohistochemistry. We performed clustering and annotation of the single-cell sequencing data and externally validated the findings using the GSE232131 single-cell dataset. By inte

SUBMITTER: Chen T 

PROVIDER: S-EPMC11648971 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature