Calmodulin regulates TRPV5 intracellular trafficking and plasma membrane abundance.

ABSTRACT: As a member of the transient receptor potential (TRP) superfamily of ion channels, TRPV5 is a unique Ca²⁺-selective channel important for active reabsorption of Ca²⁺ in the kidney. TRPV5-mediated Ca²⁺ entry into the cell is controlled by a negative feedback mechanism, in which calmodulin (CaM) blocks the TRPV5 pore upon Ca²⁺ binding. Combining microscopy techniques and biochemical assays, the present study uncovered an auxiliary role for CaM in the regulation of human (h)TRPV5 intracellular trafficking. Overexpressed hTRPV5 was mainly localised to the endoplasmic reticulum (ER) and associated with peripheral ER tubules. Limiting expression using the HEK293 TET-off system revealed that hTRPV5 trafficked through the endocytic recycling pathway. CaM

SUBMITTER: Zuidscherwoude M 

PROVIDER: S-EPMC11649520 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature