Stability of Respiratory Syncytial Virus in Nasal Aspirate From Patients Infected With RSV.

ABSTRACT:

Background

Evaluation of infectious virus titer is a challenge for respiratory syncytial virus (RSV) clinical trials because of the labile nature of RSV and rapid loss of infectivity without proper specimen handling. However, there has been no rigorous investigation into RSV stability in clinical specimens.

Methods

RSV stability was investigated by evaluating virus titers and defined as titer variation from baseline within three standard deviations of our titration assay. RSV stability in viral transport medium (VTM) at 4°C and the effect of freezing method on stability were evaluated using RSV-A2 stock. RSV stability in nasal aspirates collected in VTM at 4°C was estimated by regression analysis of virus titers measured at several time points. Stability of these specimens s

SUBMITTER: Yamamoto A 

PROVIDER: S-EPMC11649581 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature