Modeling of mRNA deadenylation rates reveal a complex relationship between mRNA deadenylation and decay.

ABSTRACT: Complete cytoplasmic polyadenosine tail (polyA-tail) deadenylation is thought to be essential for initiating mRNA decapping and subsequent degradation. To investigate this prevalent model, we conducted direct RNA sequencing of S. cerevisiae mRNAs derived from chase experiments under steady-state and stress condition. Subsequently, we developed a numerical model based on a modified gamma distribution function, which estimated the transcriptomic deadenylation rate at 10 A/min. A simplified independent method, based on the delineation of quantile polyA-tail values, showed a correlation between the decay and deadenylation rates of individual mRNAs, which appeared consistent within functional transcript groups and associated with codon optimality. Notably, these rates varied during the stress r

SUBMITTER: Czarnocka-Cieciura A 

PROVIDER: S-EPMC11649921 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature