Novel Transgenic Zebrafish Lines to Study the CHRNA3-B4-A5 Gene Cluster.

ABSTRACT: Acetylcholine (ACh), a vital neurotransmitter for both the peripheral (PNS) and central nervous systems (CNS), signals through nicotinic ACh receptors (nAChRs) and muscarinic ACh receptors (mAChR). Here, we explore the expression patterns of three nAChR subunits, chrna3, chrnb4, and chrna5, which are located in an evolutionary conserved cluster. This close genomic positioning, in a range of vertebrates, may indicate co-functionality and/or co-expression. Through novel transgenic zebrafish lines, we observe widespread expression within both the PNS and CNS. In the PNS, we observed expression of chrna3^tdTomato, chrnb4^eGFP, and chrna5^tdTomato in the intestinal enteric nervous system; chrna5^tdTomato and chrnb4^eGFP in sensory ganglia of the

SUBMITTER: Hua Y 

PROVIDER: S-EPMC11650009 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature