Depletion of intrinsic renal macrophages with moderate-to-high expression of CD163, MRC1, PTH2R, PDE4D, and CUBN in regulating podocyte injury in diabetic nephropathy: a single-cell RNA sequencing analysis.

ABSTRACT:

Background

Diabetic nephropathy (DN), a severe complication of diabetes, is characterized by glomerular and tubular damage, which often leads to end-stage renal disease (ESRD). The role of renal macrophages (Mφs), particularly their phenotypic plasticity and function in DN, remains poorly understood. This study investigated the key factors influencing Mφ polarization and their impact on podocyte (PODO) injury in DN.

Methods

Single-nuclear RNA sequencing (snRNA-seq) data from DN and control (CON) kidney samples were analyzed for cell clustering, differential expression, and cell communication. Mφs were identified and categorized based on their gene expression profiles. The proportions and functions of different Mφ phenotypes were compared between DN and CON samples, with a fo

SUBMITTER: Guo Y 

PROVIDER: S-EPMC11650352 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature