Accelerating Antimalarial Drug Discovery with a New High-Throughput Screen for Fast-Killing Compounds.

ABSTRACT: The urgent need for rapidly acting compounds in the development of antimalarial drugs underscores the significance of such compounds in overcoming resistance issues and improving patient adherence to antimalarial treatments. The present study introduces a high-throughput screening (HTS) approach using 1536-well plates, employing Plasmodium falciparum lactate dehydrogenase (PfLDH) combined with nitroreductase (NTR) and fluorescent probes to evaluate inhibition of the growth of the asexual blood stage of malaria parasites. This method was adapted to efficiently assess the speed of action profiling (SAP) in a 384-well plate format, streamlining the traditionally time-consuming screening process. By successfully screening numerous compounds, this approach identified fast-killing hits ea

SUBMITTER: Sakura T 

PROVIDER: S-EPMC11650643 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature