A comprehensive two-hybrid analysis to explore the <i>Legionella pneumophila</i> effector-effector interactome.

ABSTRACT: Legionella pneumophila uses over 300 translocated effector proteins to rewire host cells during infection and create a replicative niche for intracellular growth. To date, several studies have identified L. pneumophila effectors that indirectly and directly regulate the activity of other effectors, providing an additional layer of regulatory complexity. Among these are "metaeffectors," a special class of effectors that regulate the activity of other effectors once inside the host. A defining feature of metaeffectors is direct, physical interaction with a target effector. Metaeffector identification, to date, has depended on phenotypes in heterologous systems and experimental serendipity. Using a multiplexed, recombinant barcode-based yeast two-hybrid technology we screened fo

SUBMITTER: Mount HO 

PROVIDER: S-EPMC11651115 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature