Evaluation of a Commercial TIMS-Q-TOF Platform for Native Mass Spectrometry.

ABSTRACT: Mass-spectrometry based assays in structural biology studies measure either intact or digested proteins. Typically, different mass spectrometers are dedicated for such measurements: those optimized for rapid analysis of peptides or those designed for high molecular weight analysis. A commercial trapped ion mobility-quadrupole-time-of-flight (TIMS-Q-TOF) platform is widely utilized for proteomics and metabolomics, with ion mobility providing a separation dimension in addition to liquid chromatography. The ability to perform high-quality native mass spectrometry of protein complexes, however, remains largely uninvestigated. Here, we evaluate a commercial TIMS-Q-TOF platform for analyzing noncovalent protein complexes by utilizing the instrument's full range of ion mobility, MS, and MS/MS (bo

SUBMITTER: Panczyk EM 

PROVIDER: S-EPMC11651300 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature