Deep sequencing as a diagnostic tool in patients with suspected primary vitreoretinal lymphoma.

ABSTRACT:

Purpose

To compare the diagnostic utility of metagenomic deep sequencing (MDS) to cytology, flow cytometry and gene rearrangement by PCR in ocular samples of patients with suspected vitreoretinal lymphoma (VRL).

Methods

Patients with suspected VRL underwent ocular sampling of one or both eyes at the Emory Eye Center from September 2017 to June 2022. Ocular samples were evaluated with MDS and conventional diagnostics. MDS was performed at the Ralph and Sophie Heintz Laboratory at the F.I. Proctor Foundation. Relevant demographic and clinical data were retrospectively collected from medical records. Patients were diagnosed with VRL based on clinical assessment and conventional diagnostic testing.

Results

This study included 13 patients with suspected VRL who underwent d

SUBMITTER: Choo C 

PROVIDER: S-EPMC11652249 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature