Low-input CUT&Tag for efficient epigenomic profiling of zebrafish stage I oocytes.

ABSTRACT: Histone modification signatures mark sites of transcriptional regulatory elements and regions of gene activation and repression. These sites vary among cell types and undergo dynamic changes during development and in diseases. Oocytes produce numerous maternal factors essential for early embryonic development, which are significantly influenced by epigenetic modifications. The profiling of epigenetic modifications during oogenesis remains uniquely challenging due to the presence of numerous tightly wrapped granulosa cells. Here, we successfully established a low-input CUT&Tag (Cleavage Under Targets and Tagmentation) method tailored for zebrafish stage I oocytes. This advanced technique enables high-resolution profiling of histone modifications and DNA-binding proteins, critical for unders

SUBMITTER: Zheng Q 

PROVIDER: S-EPMC11652594 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature