Deducing formation routes of oxylipins by quantitative multiple heart-cutting achiral-chiral 2D-LC-MS.

ABSTRACT: Several oxylipins are regulators of inflammation. They are formed by enzymes such as lipoxygenases or cyclooxygenases, but also stereorandomly by autoxidation. Reversed-phase liquid chromatography-tandem-mass-spectrometry (LC-MS/MS) methods for oxylipin quantification do not separate enantiomers. Here, we combine sensitive and selective oxylipin analysis with chiral separation using two-dimensional (2D)-LC-MS/MS. By multiple heart-cutting, the oxylipin peaks are transferred onto a chiral column. 45 enantiomeric pairs of (di-)hydroxy-fatty acids are separated with full gradient elution within 1.80 min, yielding lower limits of quantification <1 pg on the column. Concentrations, as well as enantiomeric fractions of oxylipins, can be determined, even at low concentrations or at high enantiome

SUBMITTER: Kampschulte N 

PROVIDER: S-EPMC11652774 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature