Research Note: Establishment of vector system harboring duck RNA polymerase I promoter for avian influenza virus.

ABSTRACT: Reverse genetics (RG) systems are extensively utilized to investigate the characteristics of influenza viruses and develop vaccines, predominantly relying on human RNA polymerase I (pol I). However, the efficiency of RG systems for avian-origin influenza viruses may be compromised due to potential species-specific interactions of RNA pol I. In this study, we reported the polymerase activities of the duck RNA pol I promoter in avian cells and the generation of recombinant avian-derived influenza viruses using a novel vector system containing the duck RNA pol I promoter region to enhance the rescue efficiency of the RG system in avian cells. Initially, we explored a putative duck promoter region and identified the optimal size to improve the existing system. Subsequently, we established an R

SUBMITTER: Jang Y 

PROVIDER: S-EPMC11652914 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature