Establishment of feline embryonic stem cells from the inner cell mass of blastocysts produced <i>in vitro</i>.

ABSTRACT:

Introduction

The rising number of cats as pets and the growing interest in animal welfare have led to an increased need for the latest treatments in feline veterinary medicine. Among these, veterinary regenerative medicine using pluripotent stem cells is gaining significant attention. However, there have been no reports on establishing feline embryonic stem cell (ESC) lines that possess the pluripotent potential and the ability to differentiate into three germ layers.

Methods

In this study, we isolated three inner cell masses from feline in vitro-derived blastocysts and subcultured them in a chemically defined medium (StemFit AK02N). We assessed the expression of undifferentiated markers, the ability to differentiate into the three germ layers, and the karyotype struc

SUBMITTER: Yoshida T 

PROVIDER: S-EPMC11652941 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature