ALKBH5 Regulates Osteogenic Differentiation via the lncRNA/mRNA Complex.

ABSTRACT: Human adipose-derived stem cells (hASCs) are commonly used in bone tissue regeneration. The N6-methyladenosine (m⁶A) modification has emerged as a novel regulatory mechanism for gene expression, playing a critical role in osteogenic differentiation of stem cells. However, the precise role and mechanism of alkylation repair homolog 5 (ALKBH5) in hASC osteogenesis remain incompletely elucidated and warrant further investigation. Herein, we employed methylated RNA immunoprecipitation sequencing, RNA sequencing, and weighted gene coexpression network analysis to identify a key long noncoding RNA (lncRNA) in hASCs: lncRNA AK311120. Functional experiments demonstrated that lnc-AK311120 promoted the osteogenic differentiation of hASCs, while a mutation at the m⁶A central sit

SUBMITTER: Song Y 

PROVIDER: S-EPMC11653282 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature