Spatiotemporal Molecular Architecture of Lineage Allocation and Cellular Organization in Tooth Morphogenesis.

ABSTRACT: The remarkable evolution of teeth morphological complexity represents a giant leap for vertebrate. Despite its importance in life history, the understanding of spatiotemporal organization of teeth remains rudimentary. Herein, a high-resolution genome-wide molecular patterning of lineage allocation and cellular organization in tooth morphogenesis is described, constructed by integrating spatial transcriptome and single-cell RNA sequencing. Twelve spatial compartments and seventeen heterogeneous cell clusters linked to tooth morphogenic milestones are identified. Eighty-eight percent of total lineage species has already appeared in the initial tooth bud rather than the generally considered sequential emergence. A previously unrecognized sprouting-like patterning mode of the dental papilla is

SUBMITTER: Jiang S 

PROVIDER: S-EPMC11653630 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature