Leveraging Blood Components for 3D Printing Applications Through Programmable Ink Engineering Approaches.

ABSTRACT: This study proposes a tunable ink engineering methodology to allow 3D printing processability of highly bioactive but otherwise low-viscous and unprintable blood-derived materials. The hypothesis relies on improving the viscoelasticity and shear thinning behavior of platelet lysates (PL) and albumins (BSA) solutions by covalent coupling, enabling simultaneous extrusion and photocrosslinking upon filament deposition. The available amine groups on proteins (PL and BSA) are exploited for coupling with carboxyl groups present in methacrylated proteins (hPLMA and BSAMA), by leveraging carbodiimide chemistry. This reaction enabled the creation of a pre-gel from these extremely low-viscous materials (≈ 1 Pa), with precise tuning of the reaction, resulting in inks with a range of controlled viscos

SUBMITTER: Sobreiro-Almeida R 

PROVIDER: S-EPMC11653660 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature