PCBP1/2 and TDP43 Function as NAT10 Adaptors to Mediate mRNA ac⁴C Formation in Mammalian Cells.

ABSTRACT: Massive numbers of modified bases in mRNAs sculpt the epitranscriptome and play vital roles in RNA metabolism. The only known acetylated RNA modification, N-4-acetylcytidine (ac⁴C), is highly conserved across cell types and among species. Although the GCN5-related acetyltransferase 10 (NAT10) functions as an ac⁴C writer, the mechanism underlying the acetylation process is largely unknown. In this study, the NAT10/PCBP/TDP43 complex mediated mRNA ac⁴C formation in mammalian cells is identified. RNA-binding proteins (RBPs) are identified, affiliated with two different families, poly(rC)-binding protein 1/2 (PCBP1/2) and TAR DNA binding protein 43 (TDP43), as NAT10 adaptors for mRNA tethering and substrate selection. Knockdown of the adaptors resulted in decre

SUBMITTER: Jiang ZY 

PROVIDER: S-EPMC11653668 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature